| 0 µg/µl Cas9 | 1 µg/µl Cas9 | 0.5 µg/µl Cas9 | ||||||
---|---|---|---|---|---|---|---|---|---|
NC1 | NC2 | NC3 | HC4 | HC5 | HC6 | LC7 | LC8 | LC9 | |
3′ UTR | 9 | 9 | 2 | 9 | 2 | – | 1 | 1 | 10 |
5′ UTR | 3 | – | 1 | 2 | – | 1 | 1 | – | 1 |
Frameshift | 1 | – | – | 1 | – | – | – | – | – |
Intron | 2 | 2 | 2 | 1 | 1 | 1 | 2 | 3 | 2 |
Non-synonymous | – | – | – | 1 | 1 | 3 | 2 | 1 | 6 |
Start lost | – | – | – | – | 1 | – | – | – | – |
Synonymous | – | – | – | – | 1 | 1 | – | 1 | 9 |
Splice region | 1 | – | 1 | – | – | – | – | 1 | – |