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Table 3 Cas9-mediated gene deletion does not cause alterations in the types of coding region mutations in A. fumigatus

From: Whole-genome sequencing reveals highly specific gene targeting by in vitro assembled Cas9-ribonucleoprotein complexes in Aspergillus fumigatus

  0 µg/µl Cas9 1 µg/µl Cas9 0.5 µg/µl Cas9
NC1 NC2 NC3 HC4 HC5 HC6 LC7 LC8 LC9
3′ UTR 9 9 2 9 2 1 1 10
5′ UTR 3 1 2 1 1 1
Frameshift 1 1
Intron 2 2 2 1 1 1 2 3 2
Non-synonymous 1 1 3 2 1 6
Start lost 1
Synonymous 1 1 1 9
Splice region 1 1 1
  1. Shown are the numbers and types of identified mutations located within coding regions of transformants within the three experimental groups