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Table 3 Cas9-mediated gene deletion does not cause alterations in the types of coding region mutations in A. fumigatus

From: Whole-genome sequencing reveals highly specific gene targeting by in vitro assembled Cas9-ribonucleoprotein complexes in Aspergillus fumigatus

 

0 µg/µl Cas9

1 µg/µl Cas9

0.5 µg/µl Cas9

NC1

NC2

NC3

HC4

HC5

HC6

LC7

LC8

LC9

3′ UTR

9

9

2

9

2

–

1

1

10

5′ UTR

3

–

1

2

–

1

1

–

1

Frameshift

1

–

–

1

–

–

–

–

–

Intron

2

2

2

1

1

1

2

3

2

Non-synonymous

–

–

–

1

1

3

2

1

6

Start lost

–

–

–

–

1

–

–

–

–

Synonymous

–

–

–

–

1

1

–

1

9

Splice region

1

–

1

–

–

–

–

1

–

  1. Shown are the numbers and types of identified mutations located within coding regions of transformants within the three experimental groups