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Table 2 Cas9-mediated gene deletion is not associated with increased genomic mutations in A. fumigatus

From: Whole-genome sequencing reveals highly specific gene targeting by in vitro assembled Cas9-ribonucleoprotein complexes in Aspergillus fumigatus

  NC1 NC2 NC3 HC4 HC5 HC6 LC7 LC8 LC9
Average coverage 53× 62× 60× 58× 54× 61× 56× 49× 38×
Total mutations 396 342 385 345 326 307 345 366 474
Average total mutations 373 ± 28 SD 326 ± 19 SD (p > 0.05) 395 ± 69 SD (p > 0.05)
Analysis based on type of mutation identified
SNPs 371 318 363 321 314 292 326 347 439
Indels 25 24 22 24 12 15 19 19 35
Analysis based on location of mutation
Intergenic 380 (96%) 331 (97%) 379 (98%) 331 (96%) 320 (98%) 301 (98%) 339 (98%) 359 (98%) 446 (94%)
Average intergenic 363 ± 28 SD 317 ± 15 SD (p = 0.04) 381 ± 57 SD (p > 0.05)
Coding region 16 (4%) 11 (3%) 6 (2%) 14 (4%) 6 (2%) 6 (2%) 6 (2%) 7 (2%) 28 (6%)
Average coding region 11 ± 5 SD 9 ± 5 SD (p > 0.05) 14 ± 12 SD (p > 0.05)
  1. Displayed are the total and average number of mutations among the three experimental groups: no (“NC”—0 µg/µl), low (“LC”—0.5 µg/µl) and high (“HC”—1 µg/µl) levels of Cas9. For the intergenic and coding region mutation rows, the numbers in parentheses represent the percent (%) of total mutations. For the average mutations per group, the mean ± standard deviation (SD) is provided. The Student’s t test assuming unequal variance was used for statistical comparisons of the Cas9 (HC or LC) and the no-Cas9 (NC) groups and p values are presented