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Table 1 Strains used in this study

From: Whole-genome sequencing reveals highly specific gene targeting by in vitro assembled Cas9-ribonucleoprotein complexes in Aspergillus fumigatus

Strain name Background
Af293 Wild type
NC1 hygR (no Cas9)
NC2 hygR (no Cas9)
NC3 hygR (no Cas9)
HC4 ΔpksP-hygR (1 µg/µl Cas9)
HC5 ΔpksP-hygR (1 µg/µl Cas9)
HC6 ΔpksP-hygR (1 µg/µl Cas9)
LC7 ΔpksP-hygR (0.5 µg/µl Cas9)
LC8 ΔpksP-hygR (0.5 µg/µl Cas9)
LC9 ΔpksP-hygR (0.5 µg/µl Cas9)
  1. For strains names, NC = no Cas9, HC = high Cas9, and LC = low Cas9. Indicated at the right are the concentrations of Cas9 used to produce each mutant strain. hygR = hygromycin resistance cassette; pksP = polyketide synthase; ΔpksP-hygR = pksP locus replaced by hygR
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Fungal Biology and Biotechnology

ISSN: 2054-3085

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