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Table 1 Strains used in this study

From: Whole-genome sequencing reveals highly specific gene targeting by in vitro assembled Cas9-ribonucleoprotein complexes in Aspergillus fumigatus

Strain name

Background

Af293

Wild type

NC1

hygR (no Cas9)

NC2

hygR (no Cas9)

NC3

hygR (no Cas9)

HC4

ΔpksP-hygR (1 µg/µl Cas9)

HC5

ΔpksP-hygR (1 µg/µl Cas9)

HC6

ΔpksP-hygR (1 µg/µl Cas9)

LC7

ΔpksP-hygR (0.5 µg/µl Cas9)

LC8

ΔpksP-hygR (0.5 µg/µl Cas9)

LC9

ΔpksP-hygR (0.5 µg/µl Cas9)

  1. For strains names, NC = no Cas9, HC = high Cas9, and LC = low Cas9. Indicated at the right are the concentrations of Cas9 used to produce each mutant strain. hygR = hygromycin resistance cassette; pksP = polyketide synthase; ΔpksP-hygR = pksP locus replaced by hygR
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Fungal Biology and Biotechnology

ISSN: 2054-3085

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