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Table 1 Specific growth rates and biomass yields for bioreactor and BioLector cultivations of P. pastoris

From: Improved microscale cultivation of Pichia pastoris for clonal screening

  d-Glucose Glycerol
Bioreactor BioLector Bioreactor BioLector
µ (h−1) 0.22 ± 0.01 0.23 ± 0.02 0.23 ± 0.01 0.24 ± 0.01
Yx/s (g/g) 0.30 ± 0.02 0.48 ± 0.03 0.60 ± 0.02 0.60 ± 0.02
  1. 4% d-glucose or 4% glycerol were used as substrate in BSMmod pH 5.0 (BioLector: 150 mM PIPPS, 0.8 mL, 1500 rpm, 30 °C; bioreactor: 0.8 L, pH = 5.0 (NH4OH/H2SO4), DO = 30%, 30 °C). Mean values ± standard deviations of at least 3 cultivations are shown