Skip to main content

Advertisement

Fig. 6 | Fungal Biology and Biotechnology

Fig. 6

From: Improved microscale cultivation of Pichia pastoris for clonal screening

Fig. 6

Microscale cultivation of phytase secreting P. pastoris::pGAPZαB_appA under carbon-limited conditions. Clones selected in a batch-screening (compare Fig. 5) were analyzed in microscale cultivation in 0.8 mL BSMmod at pH 5.0 with 2% glycerol as batch substrate and 10% dextrin at 1300 rpm and 30 °C. 25 U/L amyloglucosidase was added after 17.7, 41.8, and 66.0 h (dashed vertical lines). a Dry cell weight concentrations calculated from the online backscatter signal; b Online measurement of dissolved oxygen concentrations; c Online measurement of pH; d Protein concentrations and phytase activities in culture supernatants. All values are corrected for evaporation. WT denotes the parental strain, P. pastoris X-33. Each clone was grown in quadruplicate, lines show mean values and error bars in d denote standard deviations

Back to article page