Fig. 5
From: Improved microscale cultivation of Pichia pastoris for clonal screening
Establishing carbon-limited conditions during microscale cultivation of P. pastoris by application of enzymatic substrate release. 0.8 mL BSMmod (150 mM PIPPS pH 5.0) with 0.5% glycerol as batch substrate and 1.8% dextrin was inoculated to OD 1.7 and incubated at 1300 rpm and 30 °C. Amyloglucosidase was added after 7 h (dashed vertical line). Dry cell weight concentrations were calculated from the online backscatter signal. Light colors around solid lines show standard deviations from three individual wells