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Fig. 4 | Fungal Biology and Biotechnology

Fig. 4

From: Improved microscale cultivation of Pichia pastoris for clonal screening

Fig. 4

Clonal screening of a library of phytase secreting P. pastoris::pGAPZαB_appA. Clones were analyzed in microscale cultivation in BSMmod with 4% d-glucose. a Protein concentrations in culture supernatants were determined using the Bradford assay. b For selected clones labeled with asterisks in a, phytase activities were determined after buffer exchange in a fluorescence assay using an artificial substrate. WT denotes the parental strain, P. pastoris X-33. Each clone was grown in triplicate, error bars show standard deviations

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