Skip to main content

Advertisement

Fig. 2 | Fungal Biology and Biotechnology

Fig. 2

From: Improved microscale cultivation of Pichia pastoris for clonal screening

Fig. 2

Comparison of buffers for microscale cultivation of P. pastoris at pH 5.0. a 100 mM potassium hydrogen phthalate pH 5.0 (1200 rpm, inoculated to OD600 = 1.0); b 150 mM PIPPS pH 5.0 (1500 rpm, inoculated to OD600 = 0.16). Cells were grown in 0.8 mL BSMmod with 4% d-glucose at 30 °C. DCW concentrations were determined experimentally and calculated from online backscatter signals. Light colors around solid lines and error bars show standard deviations from at least three individual wells

Back to article page