Fig. 2
From: Improved microscale cultivation of Pichia pastoris for clonal screening
Comparison of buffers for microscale cultivation of P. pastoris at pH 5.0. a 100 mM potassium hydrogen phthalate pH 5.0 (1200 rpm, inoculated to OD600 = 1.0); b 150 mM PIPPS pH 5.0 (1500 rpm, inoculated to OD600 = 0.16). Cells were grown in 0.8 mL BSMmod with 4% d-glucose at 30 °C. DCW concentrations were determined experimentally and calculated from online backscatter signals. Light colors around solid lines and error bars show standard deviations from at least three individual wells