Fig. 5

Co-transformation of Cas9 and the CRISPR guide RNA constructs into L. maculans by Agrobacterium-mediated transformation. Wild type strain D13 was co-transformed with T-DNAs from both constructs, and selected on media containing both hygromycin and G418. One transformant (D13-CoT) was cultured on medium containing iprodione. Two independent resistant strains were obtained, their DNA isolated, and the hos1 mutations determined by amplification and sequencing. The region incorporated into the guide RNA is highlighted in green, and adjacent PAM site in blue highlight. The two iprodione resistant strains have additional nucleotides (red font) within the hos1 gene