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Fig. 4 | Fungal Biology and Biotechnology

Fig. 4

From: Spontaneous and CRISPR/Cas9-induced mutation of the osmosensor histidine kinase of the canola pathogen Leptosphaeria maculans

Fig. 4

Development of the CRISPR/Cas9 system for targeted gene disruption in L. maculans. a Phenotype of transformants on plates with or without iprodione. Three strains derive from sequential modification of the wild type (WT) strain D5, first by transformation of the hos1 guide RNA construct (strain DV1), then transformation of the Cas9 construct (strain DV2), and lastly by selection on iprodione (strain DV3). b Alignment of sequences of hos1 from the wild type and 11 independently-created iprodione resistant mutants. On the wild type sequence the protospacer adjacent motif (PAM) is in blue highlight, region incorporated in the guide RNA in green highlight, and the KpnI restriction enzyme site used for screening is underlined. Changes in the sequence in the mutants are in red text

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