Table 2 Oligonucleotide primers with restriction sites
From: Promoters from the itaconate cluster of Ustilago maydis are induced by nitrogen depletion
Plasmid | Primers/specifications | Restriction enzymes |
|---|---|---|
pUMa43 | P otef -gfp-Tnos; ori ColE1; ampR; U. maydis ip R-locus | Â |
P tad1 -gfp | fwd: CTCGGTACCTTCGACTTGGTGGATACTGCGGCTGTTG rev: GTCGGTCTTGACTCGACCCAGCTCACCGGATCCGTA | KpnI/BamHI |
P itp1 -gfp | fwd: TCGAAATTCGAGCTCGGTACCGACCTGACAGAAGAGATAG rev: AGCTCCTCGCCCTTGCTCACCATGGTTCGACTTGGTGGATACTG | KpnI/NcoI |
P adi1 -gfp | fwd: TCGAAATTCGAGCTCGGTACCTCAGCCGATAGGTTTCAC rev: AGCTCCTCGCCCTTGCTCACCATGGTGAGCTGGGTCGAGTC | KpnI/NcoI |
P mtt1 -gfp | fwd: TCGAAATTCGAGCTCGGTACCGTTTACCGCACGCTGTA rev: AGCTCCTCGCCCTTGCTCACCATGGTGGATGACGAATCTCAAG | KpnI/NcoI |