Table 1 Pigment production by Neurospora spp. using different experimental set-ups
From: Filamentous ascomycetes fungi as a source of natural pigments
| Ascomycete | Substrate | Mode of operationb | Pigment(s) | λ max (nm)a | Pigment extraction solvent | Production | References |
|---|---|---|---|---|---|---|---|
| Neurospora crassa | 60% tapioca by product and 40% tofu waste | SSF | β-Carotene (yellow-orange) | 429, 452, 478 | THF | 295 µg/g | [13, 38, 39] |
| Neurospora crassa 74-OR23-1A | Vogel’s minimal broth | SmF, in 500-mL Erlenmeyer flask | Neurosporaxanthin | 477 | Acetone | – | [23, 40] |
| Neurospora crassa | Not defined medium with addition of 1% Bacto-Difco agar | Fernbach flasks (200 ml) for 15 days, followed by 1 day in plastic tent (oxygen atmosphere) (SSF) | Neurosporaxanthin | 477.5, 504 (in petroleum ether), 471.5, 496 (in acetone) | Acetone, petroleum ether | 0.33 mg/g dry weight | [22, 23] |
| Neurospora sitophila | Not defined medium with addition of 1 g/l Bacto-Difco yeast extract | Aerated flasks for 6 days, wet mycelial was then placed on petri dishes in a transparent plastic tent for 1 day. (SSF) | Neurosporaxanthin | 477 (petroleum ether) | Methanol, petroleum ether | 0.04 mg/g dry weight | [22, 23] |
| Neurospora intermedia N-1 | Maltose, peptone, yeast extract, Mg2+ fermentationmedia | SmF* in 1 l Erlenmeyer flask | Yellow-orange | 480 | 1 g of spores was extracted with 5 ml of acetone | 24.31 µg/g spores | [9] |
| Neurospora intermedia (PTCC 5291) | Vogel’s growth medium | SmF* in 250 ml Erlenmeyer flasks | Mixture of carotenoids | 470 | 50 mg dried mycelia were extracted in 3 ml methanol, re-extracted with 3 ml of acetone | 500 mg/l | [36] |