Fig. 2From: Effect of secretory pathway gene overexpression on secretion of a fluorescent reporter protein in Aspergillus nidulans Validation of the secretion reporter. Left Liquid cultures of NID1439 grown for 48 h at 30 °C without shaking. Middle Fluorescence microscopy of NID1439. Fluorescence localizes to plasma membrane, septa and ER-like structures. Right SDS-PAGE of His-purified (1) mRFP, cell culture supernatant from CM (2) and ladder (3). Scale bar 10 µmBack to article page