Figure 3

Rate of homologous integration at clr-2 locus in wild type with CRISPR/Cas9 technology versus mus-51 KO with the traditional method. a The number of Ignite-resistant colonies by clr-2 locus targeted transformation in the mus-51 KO and wild type (WT: 74-OR23-1V A) with CRISPR/Cas9. Error bars corresponds to the SEM. b qPCR analysis to assess the number of clr-2 in genomic DNA from the transformants with either WT (right panel) or mus-51 KO backgrounds (left panel). Error bars corresponds to the SEM. c qPCR analysis to assess the number of gh6-2 in genomic DNA from the transformants with either WT (right panel) or mus-51 KO backgrounds (left panel). Error bars corresponds to the SEM. d Schematic overview of the priming sites for PCR analysis to confirm the connection of β-tubulin promoter and clr-2. e PCR assay using β-tubulin-p F and clr-2 R primers. Expected fragment size: 1,343 bp.