Figure 4

Pcs1 is targeted to the mitochondria. (A) 10-fold serial dilutions of S. pombe strains AS09, AS12 and AS15 from top to bottom on yeast nitrogen base media with or without CdSO₄ (4 d at 22°C). The starting wild type strain is the ura4 mutant, and “empty” indicates the strain carrying the plasmid pREP42. (B) Co-localization of Pcs1-GFP in S. pombe with MitoTracker red (strain AS15). Scale bar = 10 μm.