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Table 1 Features of flanking regions of insertional mutants of Leptosphaeria maculans analysed by Illumina next-generation sequencing

From: Next-generation genome sequencing can be used to rapidly characterise sequences flanking T-DNA insertions in random insertional mutants of Leptosphaeria maculans

Mutant Plasmid inserted Number of reads (Genome coverage) Number of reads flanking each border (LB, RB) Number of nucleotides deleted from T- DNA borders (LB, RB) Number of nucleotides deleted from L. maculans DNA (bp) Genes adjacent to T- DNA insertion site
Theoretical Observed
UM176 pKHT 4361600 (14.5×) 11.6, 11.6 16, 12 261 , 4 26,312 Between Lema_G56010 and Lema_G101830b
UM314a pKO21 7219200 (24×) 19.3, 19.3 12, 11 43, 44 16 Within Lema_G051670
UM316 pKO21 1985280 (6.6×) 5.3, 5.3 4, 0 87, RB deleted Nd Between Lema_G008960 and Lema_G008970
UM317 pKO21 3308800 (11×) 8.8, 8.8 10, 3 342, 42 22 Between Lema_G001330 and Lema_G001340
  1. aUM314 had two T-DNA insertion sites; however, only one was identified in this study.
  2. bTranslocation between Supercontig 5 and Supercontig 17 associated with T-DNA insertion.
  3. Nd = Not determined.