Next-generation genome sequencing can be used to rapidly characterise sequences flanking T-DNA insertions in random insertional mutants of Leptosphaeria maculans

Table 1 Features of flanking regions of insertional mutants of Leptosphaeria maculans analysed by Illumina next-generation sequencing

Mutant	Plasmid inserted	Number of reads (Genome coverage)	Number of reads flanking each border (LB, RB)		Number of nucleotides deleted from T- DNA borders (LB, RB)	Number of nucleotides deleted from *L. maculans* DNA (bp)	Genes adjacent to T- DNA insertion site
Mutant	Plasmid inserted	Number of reads (Genome coverage)	Theoretical	Observed	Number of nucleotides deleted from T- DNA borders (LB, RB)		Genes adjacent to T- DNA insertion site
UM176	pKHT	4361600 (14.5×)	11.6, 11.6	16, 12	261 , 4	26,312	Between Lema_G56010 and Lema_G101830^b
UM314^a	pKO21	7219200 (24×)	19.3, 19.3	12, 11	43, 44	16	Within Lema_G051670
UM316	pKO21	1985280 (6.6×)	5.3, 5.3	4, 0	87, RB deleted	Nd	Between Lema_G008960 and Lema_G008970
UM317	pKO21	3308800 (11×)	8.8, 8.8	10, 3	342, 42	22	Between Lema_G001330 and Lema_G001340

^aUM314 had two T-DNA insertion sites; however, only one was identified in this study.
^bTranslocation between Supercontig 5 and Supercontig 17 associated with T-DNA insertion.
Nd = Not determined.

ISSN: 2054-3085