Figure 4

De novo synthesis of amino acids in P. sapidus . The bars represent the relative amount of de novo synthesized amino acids during growth in standard nutrition solution (SNL-H3-G15) and two chemical defined media with different aspartate concentrations (0.4 g L-1: NL-D0.4-G15; 4.8 g L-1: NL-D5-G15). The contribution of de novo synthesis was estimated from the amount of label measured in the amino acids, which originated from 50% [U-¹³C]-labeled glucose as main carbon source. Error bars represent the range of duplicates.