Table 3 The primers used in this study
Name | Sequence 5′-3′ | Aplication |
|---|---|---|
attB1_TrpC_CFP_F | ggggacaagtttgtacaaaaaagcaggct ATGGACGAGCTGTACAAGTAA | Amplification TrpC-pyrG-TrpC cassette |
attB2r_TrpC_R | ggggaccactttgtacaagaaagctgggt TGGGTGTTACGGAGCATTCACTAGGC | Amplification TrpC-pyrG-TrpC cassette |
attB4_ugeA5F | ggggacaactttgtatagaaaagttg CCGATAGGAAGGATGAGGAT | Amplification 5′flank ugeA disruption cassette |
attB1r_ugeA5R | ggggactgcttttttgtacaaacttg GATGATGATAAGGTATGACT | Amplification 5′flank ugeA disruption cassette |
attB2r_ugeA3F | ggggacagctttcttgtacaaagtgg ATAATGACCCCGCATATGTT | Amplification 3′flank ugeA disruption cassette |
attB3_ugeA3R | ggggacaactttgtataataaagttg GGATGGACAGCCGTGCAGTG | Amplification 3′flank ugeA disruption cassette |
ugmAP1f-NotI | aaggaaaaaagcggccgc AGGACTCCATAGGCCCGTAGA | Amplification ugmA for complementation and sequencing |
ugmAP2r-NotI | aaggaaaaaagcggccgc AGAAACGGACTGCATGGGC | Amplification ugmA for complementation and sequencing |
ugeAfw-SmaI | ctcgagcccggg TTGATTGGACCCTTGGGATCG | Amplification ugeA for complementation and sequencing |
ugeArev-SmaI | ctcgagcccggg TTGGCAAGGAAGGAGGTGAAG | Amplification ugeA for complementation and sequencing |