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Table 2 Aspergillus niger strains used in this study

From: Identification of the UDP-glucose-4-epimerase required for galactofuranose biosynthesis and galactose metabolism in A. niger

Strain

Description

References

N402

cspA1 derivative of ATCC9029

Bos et al. 1988

MA70.15

ΔkusA::amdS, pyrG-

[25]

MA169.4

ΔkusA::DR_amdS_DR, pyrG-

[18]

MA234.1

MA169.4 transformed with pAB4.1 to make strain pyrG+

Arentshorst, unpublished

RD6.47

pPagsA-amdS-TamdS-pyrG* and pPagsA-H2B-GFP-TtrpC/pAN7.1

[8]

RD6.47#41

galF- mutant derived from RD6.47

[8]

RD6.47#41, pyrG-

pryG- derivative of RD6.47#41

This study

RD6.47#41/pAMA_ugeA

RD6.47#41/pyrG- pBlue_AMA_ugeA

This study

RD6.47#41/pAMA_ugmA

RD6.47#41/pyrG- pBlue_AMA_ugmA

This study

RD6.47#41/pAMA

RD6.47#41/pyrG- pBlue_AMA

This study

JH12.1

ugeA (An14g03820)::pyrG deletion in MA169.4

This study

JH21.1

pyrG- derivative of JH12.1

This study

JH21.1.1

ΔugeA/pAMA_ugeA

This study

JH21.1.2

ΔugeA/pAMA_ugmA

This study

JH21.1.3

ΔugeA/pAMA

This study

MA87.6

ugmA (An02g08660)::pyrG in MA70.15

[8]

MA247.2.1b

pryG- derivative of MA87.6

This study

MA247.2.1b.1

ΔugmA/pAMA_ugeA

This study

MA247.2.1b.2

ΔugmA/pAMA_ugmA

This study

MA247.2.1b.3

ΔugmA/pAMA

This study

RD15.4

pPagsA-H2B-GFP-TtrpC-pyrG* and pPagsA-amdS-TamdS/pAN7.1

[8]

RD15.4#5

galF- mutant derived from RD15.4

[8]

RD15.4#18

galF- mutant derived from RD15.4

[8]

RD15.4#30

galF- mutant derived from RD15.4

[8]

RD15.4#50

galF- mutant derived from RD15.4

[8]

RD15.4#57

galF- mutant derived from RD15.4

[8]

RD6.13#44 (miaA)

ugmA mutant derived from RD6.13

[8]

RD15.4#17 (miaB)

ugmA mutant derived from RD15.4

[8]

RD6.13#50 (miaC)

ugmA mutant derived from RD6.13

[8]

  1. pyrG+ = pyrG plus (uridine prototroph); pyrG- = pyrG minus (uridine auxotroph); pyrG* = mutant pyrG allele for targeting at the pyrG locus).