Skip to main content

Advertisement

Figure 5 | Fungal Biology and Biotechnology

Figure 5

From: Engineering of Aspergillus niger for the production of secondary metabolites

Figure 5

Optimization of the enniatin yield. 5 × 106 spores/ml of strain DS3.1 were cultivated in 20 ml shake flask cultures containing complete medium with varying composition. Induction of esyn1 expression was performed in all media with 10 μg/ml Dox. Selected results are exemplarily shown: (1) 0 mM l-valine/l-isoleucine/l-leucine, 1% glucose, 0 g/l talcum, 40 h cultivation time, 30°C cultivation temperature. (2) 10 mM l-valine/l-isoleucine/l-leucine, 1% glucose, 0 g/l talcum, 10 mM d-Hiv, 92 h cultivation time, 26°C cultivation temperature. (3) 10 mM l-valine/l-isoleucine/l-leucine, 2.5% glucose, 2.5 g/l talcum, 10 mM d-Hiv, 92 h cultivation time, 26°C cultivation temperature. (4) 20 mM l-valine/l-isoleucine/l-leucine, 5% glucose, 10 g/l talcum, 10 mM d-Hiv, 92 h cultivation time, 26°C cultivation temperature. The total enniatin concentration (black bars) and biomass concentration (grey bars) is given. Data from biological triplicates are shown. Microscopic pictures of DS3.1 pellets are shown. Bar, 500 μm.

Back to article page