Figure 5

Optimization of the enniatin yield. 5 × 10⁶ spores/ml of strain DS3.1 were cultivated in 20 ml shake flask cultures containing complete medium with varying composition. Induction of esyn1 expression was performed in all media with 10 μg/ml Dox. Selected results are exemplarily shown: (1) 0 mM l-valine/l-isoleucine/l-leucine, 1% glucose, 0 g/l talcum, 40 h cultivation time, 30°C cultivation temperature. (2) 10 mM l-valine/l-isoleucine/l-leucine, 1% glucose, 0 g/l talcum, 10 mM d-Hiv, 92 h cultivation time, 26°C cultivation temperature. (3) 10 mM l-valine/l-isoleucine/l-leucine, 2.5% glucose, 2.5 g/l talcum, 10 mM d-Hiv, 92 h cultivation time, 26°C cultivation temperature. (4) 20 mM l-valine/l-isoleucine/l-leucine, 5% glucose, 10 g/l talcum, 10 mM d-Hiv, 92 h cultivation time, 26°C cultivation temperature. The total enniatin concentration (black bars) and biomass concentration (grey bars) is given. Data from biological triplicates are shown. Microscopic pictures of DS3.1 pellets are shown. Bar, 500 μm.