Analysis of enniatins produced by strain DS3.1. A) HR-LCMS chromatogram of purified enniatin B. A segment from the mass spectrum shows [M + H+] of enniatin B with the characteristic isotope pattern. The main peak can be assigned to enniatin B. Minor impurities can be detected at retention time 11.8 min and 12.2 min. B) HR-LCMS average mass spectrum. As example, the mass spectrum of purified enniatin B is shown. The H+, NH4
+, Na+ adducts of enniatin B can be observed. The sample contains small amounts of enniatin B1. Samples were measured on an ESI-Orbitrap-MS. C) ESI-HRMS/MS spectrum obtained with a LTQ Orbitrap XL apparatus using direct injection and applying a collision energy of 12 eV. The moiety highlighted in green represents the l-valine and the moiety highlighted in red represents d-Hiv incorporated into the enniatin B structure. For the fragments m/z values were calculated. The calculated m/z value for the [C27H47N2O8]+ fragment is 527.33269 and the m/z value observed was 527.33270. For the [C22H39N2O6]+ fragment, the calculated m/z value was 427.28026 and the m/z value observed was 427.28021. The calculated m/z value for the [C16H28NO5]+ fragment was 314.19620 and the m/z value measured was 314.19638. The calculated m/z value for the [C11H20NO3]+ fragment was 214.14377 and the m/z value observed was 214.14392.