RNA-sequencing reveals the complexities of the transcriptional response to lignocellulosic biofuel substrates in Aspergillus niger

Table 1 Expression of key CAZy-encoding genes that are induced on the willow substrate

Gene ID		Annotation ^a	CAZy family		RPKM
ATCC 1015	CBS513.88			Glucose 48 h	Willow ^b 24 h	Willow 24 h + Glucose 5 h
TID_211595	An12g04610	Lytic polysaccharide monooxygenase	AA9	0.6	246.9	1.2
TID_209376	An07g08950	eglB - Heat- and alkaline-stable endoglucanase	GH5	0.5	210.1	0.7
TID_211544	An12g05010	axeA - Acetyl xylan esterase	CE1	0.6	161.5	0.3
TID_53159	An07g09330	cbhA - Putative cellobiohydrolase	GH7	1.0	155.7	1.2
TID_54490	An12g02220	Putative cellulose 1,4-beta-cellobiosidase	CBM1, GH6	0.3	145.2	0.4
TID_205580	An01g11670	Endoglucanase A	GH5, CBM1	0.2	120.6	1.6
TID_211053	An14g02760	eglA - Putative secreted endoglucanase A	GH12	0.4	116.3	1.4
TID_51773	An01g11660	cbhB - Putative cellobiohydrolase B	GH7, CBM1	0.3	115.9	10.8
TID_133986	An08g01760	Putative cellulase; exocellobiohydrolase	GH6	0.2	54.9	0.5
TID_52011	An01g03340	Xyloglucan-specific endo-beta-1,4-glucanase	GH12	0.5	49.3	0.7

^aThe annotations here are for the A. niger CBS513.88 strain from the AspGD database [44] with the exception that the erroneous endoglucanase annotation for the AA9 family member was corrected.
^bAll of the genes listed here have significantly increased expression on the willow substrate compared to the glucose 48 h control (p < 0.05, DESeq).

ISSN: 2054-3085