Fig. 5
From: Genetic regulation of l-tryptophan metabolism in Psilocybe mexicana supports psilocybin biosynthesis
Chromatographic analysis of IasA activity assays to detect tryptophol formation by P. mexicana IasA. (A) Chromatograms were extracted at λ = 280 nm. Top trace a: overlaid chromatograms of l-tryptophan and tryptophol references, center trace b: reaction with IasA, bottom trace c: negative control with heat-inactivated IasA. (B) Extracted ion chromatograms (EICs; m/z 162 and 205 [M + H]+). (C) Mass spectra of chromatographic signals of l-tryptophan (*) and tryptophol (**) in trace b, recorded in positive mode