Fig. 4

Substrate specificity of P. mexicana IasA. Photometric detection of hydrazone formation from IasA-produced aldehydes and 2,4-dinitrophenylhydrazine (2,4-DNPH). Absorption was measured at λ = 500 nm and 800 nm (reference wavelength). The value of the heat-inactivated control thus obtained was subtracted from the respective value of the reactions with native enzyme. The experiment was performed with two biological replicates and three technical replicates each. Mean values and standard deviations are shown