Table 2 Quantitative analysis of submerged cultivations using µCT
Equivalent pellet diameter [µm] | Hyphal diameter [µm] | Total hyphal length [m] | Number of tips [-] | Number of branches [-] | Hyphal branch unit (HBU) [µm] | Hyphal growth unit (HGU) [µm] | ||||
|---|---|---|---|---|---|---|---|---|---|---|
MJK17.25 (control, n = 654) | 512 ± 157 | 3.08 ± 0.06 | 0.37 ± 0.40 | 3,834 ± 3,830 | 2,267 ± 2,428 | 159 ± 11.1 | 90 ± 14.0 | |||
LB7.1 (ΔcsmB, n = 472) | 528 ± 172 | 3.06 ± 0.06 | 0.36 ± 0.52 | 4,651 ± 6,305 | 2,247 ± 3,098 | 157 ± 9.6 | 74 ± 8.5 | |||
LB8.18 (ΔcsmA, n = 177) | 753 ± 234 | 3.04 ± 0.05 | 0.92 ± 0.79 | 11,899 ± 9,959 | 5,983 ± 5,115 | 155 ± 11.8 | 77 ± 9.7 | |||
LB9.1 (ΔchsF, n = 189) | 750 ± 231 | 3.07 ± 0.11 | 1.12 ± 1.23 | 15,060 ± 14,708 | 7,784 ± 8,192 | 142 ± 22.8 | 73 ± 32.0 | |||
LB10.1 (ΔchsA, n = 663) | 422 ± 157 | 3.06 ± 0.06 | 0.19 ± 0.29 | 2,471 ± 3,539 | 1,262 ± 1,904 | 152 ± 12.0 | 75 ± 11.3 | |||
LB11.1 (ΔchsG, n = 653) | 490 ± 160 | 3.08 ± 0.08 | 0.28 ± 0.30 | 3,216 ± 3,078 | 1,769 ± 1,860 | 155 ± 13.5 | 82 ± 15.7 | |||
LB12.14 (ΔchsD, n = 716) | 445 ± 176 | 3.09 ± 0.09 | 0.23 ± 0.35 | 2,684 ± 3,644 | 1,438 ± 2,130 | 160 ± 15.2 | 80 ± 15.4 | |||
LB13.3 (ΔchsB, n = 86) | 965 ± 269 | 3.10 ± 0.05 | 2.59 ± 1.83 | 19,533 ± 14,700 | 16,074 ± 11,374 | 162 ± 7.4 | 132 ± 25.2 | |||
LB14.1 (ΔchsE, n = 686) | 491 ± 145 | 3.02 ± 0.04 | 0.38 ± 0.41 | 4,017 ± 4,057 | 2,294 ± 2,479 | 166 ± 8.6 | 91 ± 12.7 | |||
SD1.3 (ΔchsC, n = 228) | 601 ± 205 | 3.00 ± 0.04 | 0.45 ± 0.47 | 5,657 ± 5,763 | 2,848 ± 2,977 | 156 ± 11.0 | 77 ± 10.2 | |||
ME1.4 (ΔchsE + ΔchsD, n = 628) | 523 ± 156 | 3.02 ± 0.05 | 0.40 ± 0.48 | 4,008 ± 4,587 | 2,461 ± 2,906 | 164 ± 9.9 | 99 ± 14.7 | |||