Table 2 List of candidate reference genes and target genes used in the RT-qPCR analysis
Gene symbol | Accession | Primer sequence (5’-3’) | Amplicon length [bp] | Tm [\(^{\circ }\hbox {C}\)] | Final concentration [nM] |
|---|---|---|---|---|---|
act1 | 420323 | F: AGGTAGGCGTGACTTGAACA R: TGAGCTTGGCACGAGTGATT | 122 | F: 58.95 R: 59.96 | 400 |
btl | 422722 | F: ATGGCTGCTTCTGACTTCCG R: TGTTCTGCACGTTTCGCATC | 166 | F: 60.39 R: 59.77 | 400 |
fis1 | 303068 | F: TACAAGCTCGGCAACTACGG R: CGACCTTGTCGTCGATGAGT | 112 | F: 60.11 R: 59.83 | 400 |
gapdh | 443719 | F: CGAGGAGATTACCAACGCCA R: GTTGTTGTTGCCGAGCATGT | 167 | F: 59.83 R: 59.97 | 400 |
lox1 | 330921 | F: GCCTTCTTACCGAAGCCA R: CTGCCAGCATCCTTGGAA | 200 | F: 56.97 R: 57.25 | 400 |
pks4 | 489002 | F: AGCGACGACTACAGAGAGGT R: TGTCACATTCATTGCGCCAG | 202 | F: 60.04 R: 59.48 | 400 |
rho | 331454 | F: TCAACTTCACCAGCCCACTG R: GGTCGAGTTCGAGCAATCCT | 153 | F: 60.18 R: 59.83 | 400 |
rpl6e | 414343 | F: AGGCTTTCTTCAAGCAGGGA R: CGGCTGCTGTTGATCTCCT | 168 | F: 59.23 R: 59.78 | 400 |
sar1* | 457369 | F: CTCGACAATGCCGGAAAGACCA R: TTGCCAAGGATGACAAAGGGG | 305 | F: 63.12 R: 60.83 | 400 |
tbp | 444185 | F: GAAGACGATTGCTCTGCACG R: ATGCGAAGATGAGGGCAGTT | 184 | F: 59.63 R: 59.75 | 400 |
tef1 | 419042 | F: CACCATCGACATTGCCCTCT R: CAGTCAGCCTGGGAAGTACC | 182 | F: 60.11 R: 59.75 | 400 |
vma1 | 173248 | F: ACTGGATGAACTGGACCGTG R: TCTCGCTGCTTTTTGTTGGC | 130 | F: 59.68 R: 59.97 | 200 |