Table 1 Amplification efficiencies, correlation coefficients (\(\text {R}^2\)) and slope of RT-qPCR primers of ten candidate reference genes
Candidate reference gene | Amplification efficiency | \(\text {R}^2\) | Slope |
|---|---|---|---|
act1 | 97.21 | 0.995 | − 3.1695 |
btl | 104.78 | 1.000 | − 3.2123 |
fis1 | 96.57 | 0.988 | − 3.4068 |
gapdh | 100.16 | 0.993 | − 3.3180 |
rho | 90.26 | 0.991 | − 3.5798 |
rpl6e | 106.08 | 0.990 | − 3.1842 |
sar1 | 109.33 | 0.999 | − 3.1168 |
tbp | 106.17 | 0.999 | − 3.1824 |
tef1 | 109.87 | 0.999 | − 3.1060 |
vma1 | 109.76 | 0.997 | − 3.1082 |