Fig. 1

Expression variability of each of the ten tested reference gene candidates among both tested light conditions, light–dark cycle and complete darkness (dark–dark), presented as box-and-whisker plot. The line across the box indicates the median value. The box indicates the 25th and 75th percentiles, whereas the whisker represents the maximum and minimum values. A Raw expression of each gene in T. atroviride cultivated under light–dark conditions shown as Cq values. B Raw expression of each gene in T. atroviride cultivated under complete darkness shown as Cq values