Table 2 Details of primers used for the cloning of sgRNA and quantitative RT-PCR
Gene Name | Accession Number | Sequences (5′–3′) of paired primers | Product Size (bp) |
|---|---|---|---|
ACE1 | Talem1p7_004885 | ATCGCCAAGAGGAAGACG/ATGATGCGCTGTCTGGA | 146 |
CreA | Talem1p7_016699 | AGACACATTCGGACGCATC/TGTGCTGTGCCTTGTTACT | 148 |
XlnR | Talem1p7_014973 | CGATCAACATGCTACGCAATC/GGTGACGATCCATCACATAGAG | 129 |
LPMO (AA9) | Talem1p7_002983 | GCTGTTTCTTCTACTGCTCCT/TTCGATCTCCTCCGCAATAAC | 123 |
β-Glucosidase A (GH3) | Talem1p7_000240 | GCTTCGACCGTATCACACTT/TAGTTGGTCACAACCCAGTTC | 115 |
Endoglucanse-B (GH5_5) | Talem1p7_004873 | CTCGACTATCTCGCCAACAATAC/AGAACCTGCTCATACGCAATC | 125 |
Endoglucanase (GH5_4) | Talem1p7_014763 | CTTCACTACCGTTCCTCTTCTG/TAGCGGTGCGGATGAAATAG | 104 |
Endoglucanase (GH7) | Talem1p7_015491 | GTCCCAACGAAGAGGAATGT/CCGTTCGTGACGTACTGATT | 115 |
CellobiohydrolaseC (GH6) | Talem1p7_000760 | ACCTGAACGTGGCGAAAT/GCGTGTCCAGCATCAAGATA | 115 |
Xylanase (GH10) | Talem1p7_010333 | CCAGATGTTGCGGTGTCATA/TGGCCCTTGTAATGGGTAAC | 138 |
β-Xylosidase (GH3) | Talem1p7_009060 | GACCCAGAGCATGTCAAGAT/ATCCTGCTGCGTGATGATAG | 114 |
α-Mannosidase (GH47) | Talem1p7_011066 | CCCATCTCTGTCACTCTCTTTG/TTATACCCGTCCCAAGCAATC | 144 |
EF-1 | Talem1p7_017962 | CCAGGGTCCCAAGAAAGAAA/TACCCAGGCCTAGCTTCTTA | 111 |
VS_ACE1-F-902 | Talem1p7_004885 | ACCTTCCUGTCCCCAGGTTTTAGAGCTAGAAATAGCAAGTTAAA | –- |
VS_ACE1-R-902 | AGGAAGGUCGTCTGCATCATCCGTGAATCGAAC | –- | |
VS_CSN438-F | –- | GGGTTTAAUGATCACATAGATGCTCGGTTGACA | –- |
VS_CSN790-R | –- | GGTCTTAAUACCCTGAGAAGATAGATGTGAATGTG | –- |
ACE1-sgRNA | –- | GACGACCTTCCTGTCCCCAG | –- |