CRISPR/Cas9 mediated gene editing of transcription factor ACE1 for enhanced cellulase production in thermophilic fungus Rasamsonia emersonii

Table 1 Screening of gene edited transformants for cellulase and xylanase enzyme activities

Activity against different substrates (Units/ml)
Mutants	Endoglucanase^a	Cellobiohydrolase (CBHI)^b	β-glucosidase^c	Fpase^d	Xylanase^e
M36	446.88 ± 7.12	11.11 ± 0.55	53.24 ± 1.12	4.02 ± 1.62	306.27 ± 3.27
GN1	403.87 ± 5.18	11.13 ± 0.51	52.81 ± 1.67	3.60 ± 1.11	303.13 ± 5.44
GN2	470.40 ± 6.25	10.99 ± 1.10	44.66 ± 0.99	4.02 ± .89	281.80 ± 3.66
GN3	457.63 ± 5.14	10.30 ± .087	51.94 ± 0.58	4.00 ± .75	284.34 ± 4.12
GN4	455.61 ± 6.16	10.42 ± .043	50.21 ± 0.88	3.88 ± 1.44	274.95 ± 4.68
GN6	444.19 ± 6.54	10.26 ± 1.02	45.35 ± 1.16	4.01 ± 1.61	295.56 ± 5.99
GN11	520.12 ± 3.16	15.34 ± 0.94	65.90 ± 2.08	4.46 ± 0.79	370.78 ± 3.58
Mix2	461.66 ± 8.34	10.40 ± 1.43	50.21 ± 3.32	3.67 ± .92	308.14 ± 4.46
Mix3	424.70 ± 5.78	10.42 ± 0.45	45.09 ± 2.66	3.98 ± .43	291.63 ± 3.14
Mix5	499.29 ± 4.89	14.11 ± 0.17	60.79 ± 1.94	4.35 ± 0.31	350.73 ± 3.14
Mix6	442.84 ± 5.16	10.89 ± .053	46.39 ± 2.47	4.05 ± 1.17	279.06 ± 4.57

Data expressed as mean values (±SE), from three independent repeats
The enzyme activities were estimated against different substrates (a, Carboxymethyl cellulose (CMC); b, p-nitrophenyl-b-D-cellobioside (pNPC); c, p-nitrophenyl-b-D-glucoside (pNPG); d, filter paper, e, birchwood xylan)

ISSN: 2054-3085