Fig. 2From: CRISPR/Cas9 mediated gene editing of transcription factor ACE1 for enhanced cellulase production in thermophilic fungus Rasamsonia emersoniiVerification of ACE1 disruption in selected transformants A Target sequence (sgRNA) highlighted in blue from first exon of ACE1 gene B Sequence alignment of the parental strain M36 and ∆ACE1 mutants (GN11 and Mix5), sgRNA is highlighted in purple. Yellow letters depict the protospacer adjacent motif (PAM)Back to article page