Fig. 4
From: Basidiomycete non-reducing polyketide synthases function independently of SAT domains
In vivo activity assays of ΔSAT-PKS of various mushrooms in Aspergillus niger. Chromatograms were recorded at λ = 254 nm. The top lane represents an authentic standard of orsellinic acid (3). Lower lanes: A. niger expressing the genes for full-length ArmB (A. niger tNAL057), ArmBΔSAT (tNAL058), PKS1ΔSAT (tNAL063), and PKS2ΔSAT (tNAL064). A. niger tNAL000 [22], containing the insertless expression vector phis_SM-Xpress_URA, and the parental strain ATNT16ΔpyrGx24 were included as negative controls to monitor the metabolic background of the host