Fig. 6
From: Rapid and robust squashed spore/colony PCR of industrially important fungi
Characterization of the effects of pigments on Squash-PCR in fungi. A The phenotype of albA transformants. B Squash-PCR using black spores (1 and 3) and white spores (2 and 4) without dilution of template, with primers OGY11/ OGY12. C Squash-PCR using black spores (1 and 3) with fivefold dilution of template DNA, with primers OGY11/ OGY12. D Spore pictures of A. niger, A. Oryzae ATCC 12892, A. Oryzae ATCC 7252 and Penicillium melinii ATCC 13352. E Spore PCR using the spores from D, with primers ITS1/D2. N, negative control; P, positive control; 1, A. niger; 2, A. Oryzae ATCC 12892; 3, A. Oryzae ATCC 7252; 4, Penicillium melinii ATCC 13352. Two technique replicates were performed for Squash-PCR