Fig. 4
From: Rapid and robust squashed spore/colony PCR of industrially important fungi
Examination of potential limiting factors that affects Squash-PCR in A.niger. A PCR to test the efficiency of different DNA polymerases in amplifying PCR products using Squash-PCR with primers ITS1/D2 and ITS1/ITS4. P, positive control; Taq, Taq DNA polymerase; Q5, Q5 High-Fidelity DNA polymerase; Phusion, Phusion High-Fidelity DNA polymerase. B The size range of PCR products effectively amplified using Q5 High-Fidelity DNA polymerase in Squash-PCR. P, positive control. C The effective range of spore concentration in Squash-PCR. N, negative control; P, positive control. D PCR to test the effects of spore age in Squash-PCR. N, negative control; P, positive control