Fig. 4

A Domain organization was analysed using the NCBI Conserved Domain [36], InterPro [37], SBSPKSv2 [38]; and the PKS/NRPS Analysis Web-site [39]. KS (red), keto synthase; AT (yellow), acyltransferase; DH (pink), dehydratase; MT (blue) C-methyltransferase; ER (grey) enoylreductase; KR (violet), ketoreductase; ACP (green), acyl carrier protein; cAT (orange), carnitine acyltransferase; Chalcone/stilbene_synt_N (lime-green): Chalcone/stilbene synthase N-terminal; Chalcone/stilbene_synt_C (dark-cyan): Chalcone/stilbene synthase C-terminal. The proposed PKS names are listed together with their gene number and length. The transcriptional activity at 24 h or 48 h ins indicated as “−” (No transcription at given timepoint) or “+” (transcription at given timepoint). The two PKS encoding genes that are proposed to be involved in the rasfonin synthesis (i.e. CgPKS4 and CgPKS5) are highlighted by a red box B The proposed BGC for rasfonin biosynthesis is depicted with transcript at 24 h and 48 h timepoint (shake flask culture, AMM). The BGC borders are depicted in red and were assigned based on the coregulation of genes. Proposed BGC gene names (rsf1 to rsf9) are depicted above gene illustration