Fig. 1From: Stable overexpression and targeted gene deletion of the causative agent of ash dieback Hymenoscyphus fraxineusDrawing of the strategy for the replacement of the hph gene of mutant #1.12 by the nptII gene. A The final construct with the nptII resistance cassette (pIGPAPAGenGFP) and B clipped part of the pIGPAPAHygGFP. Flanking regions are indicated as crossed boldface black lines. Prior to transformation, the construct was excised PvuI from the plasmid pIGPAPAGenGFPBack to article page