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Table 2 Mutations in DbURA9 expressing strains, evolved for anaerobic pyrimidine prototrophy

From: Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis

Evolution line

Strain

Mutations

Vps1

Fum1

Flask 1

IMS1167

I410L

M432I

IMS1168

I410L

M432I

Flask 2

IMS1169

–

A294V

IMS1170

–

I218M

  1. Two cultures of IMI439 (ura1Δ::DbURA9) were independently evolved under anaerobic conditions on SMUD (Additional file 5: Figure S1). Two single colony isolates from each flask were subjected to whole-genome resequencing and predicted amino-acid substitutions were only found in Vps1 and Fum1
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Fungal Biology and Biotechnology

ISSN: 2054-3085

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