Fig. 4

Z. tritici EV proteins group with EV proteins from other fungi based on sequence similarity. The fungi compared in the plot are S. cerevisiae (Sc), H. capsulatum (Hc), C. albicans (Ca), C. neoformans (Cn), F. oxysporum f. sp. vasinfectum (Fov) and P. brasiliensis (Pb). Proteins previously identified in fungal EVs were grouped into orthologous groups using Orthofinder; only intersections including Z. tritici were represented in the plot. a shows the number of orthogroups shared by all species; for example, 3 orthogroups are shared by all seven species. b Bar plot showing the number of orthogroups shared by Z. tritici and each fungal species. The intersection of Z. tritici orthogroups with those of the other fungi is represented with an Upset plot, shown in c. The bottom panel plots the intersections of orthogroups from each species. Each row represents a species and each column indicates which combination of species share x number of orthogroups. Dark, connecting dots indicate which species in a column share orthogroups. The bar plot at the top represents the size of the intersection, or how many orthogroups are shared by each combination of species. For example, 48 orthogroups are shared by Z. tritici (Zt) and Sc, Hc and Ca, while two orthogroups are species-specific to Zt