Table 3 Synthetic genes used in this study
From: Evaluation of synthetic formaldehyde and methanol assimilation pathways in Yarrowia lipolytica
Gene | Name | NCBI accession number | Origin | Optimised by/with | Optimised for |
|---|---|---|---|---|---|
Dhas | CbDhas | AAC83349 | Candida boidinii | Geneart | Y. lipolytica |
Hps | BmHps | WP_003349277 | Bacillus methanolicus | Geneart | Y. lipolytica |
Hps | BmHps_native | WP_003349277 | Bacillus methanolicus | Not optimised | Y. lipolytica |
Hps | BmHps_Sc | WP_003349277 | Bacillus methanolicus | Geneart | S. cerevisiae |
Phi | BmPhi | EIJ79730 | Bacillus methanolicus | Geneart | Y. lipolytica |
Phi | BmPhi_Sc | EIJ79730 | Bacillus methanolicus | Geneart | S. cerevisiae |
HpsPhi phusion | MgHpsPhi | AB034913 | Mycobacterium gastri | Geneart | Y. lipolytica |
Hps | BgHps | WP_053604812 | Bacillus gobiensis | Optimizer | Y. lipolytica |
Hps | BpHps | WP_040374929 | Bacillus psychrosaccharolyticus | Optimizer | Y. lipolytica |
Hps | BsHps | ASB91925 | Bacillus subtilis | Optimizer | Y. lipolytica |
Hps | HmHps | WP_020225866 | Holdemania massiliensis | Optimizer | Y. lipolytica |
Hps | LeeHps | WP_028376164 | Leeuwenhoekiella sp Hel_I_48 | Optimizer | Y. lipolytica |
Hps | LbHps | WP_034981480 | Lactobacillus wkB10 | Optimizer | Y. lipolytica |
Hps | MaHps | BAA83096 | Methylomonas aminofaciens | Optimizer | Y. lipolytica |
Hps | MvHps | WP_031433509 | Methylomarinum vadi | Optimizer | Y. lipolytica |
Hps | RtHps | WP_045855207 | Raoulterra terrigena | Optimizer | Y. lipolytica |