Table 1 Strains used in this study
Name | Genotype | Gene(s) affected | Description | Source |
|---|---|---|---|---|
NID1 | argB2, pyrG89, veA1, nkuAΔ | nkuAΔ for efficient gene targeting | Parental strain used to construct mRFP secreting strain | IBT collection #29539 [33] |
NID3 | argB2, pyrG89, veA1, nkuA-trS::AFpyrG | Transient small repeat in nkuA | Reference strain | IBT collection #28738 [33] |
NID912 | argB2, pyrG89, veA1, nkuAΔ, IS1::PgpdA::RFP::TtrpC::pyrG | Intracellular mRFP expression | Negative control | Our lab |
NID1439 | argB2, pyrG89, veA1, nkuAΔ, IS1::PgpdA-ASNglaA-mRFP-TtrpC::AFpyrG | mRFP secretion | Strain secreting mRFP | This study |
NID1595 | argB2, pyrG89, veA1, nkuAΔ, IS1::PgpdA-ASNglaA-mRFP-TtrpC | mRFP secretion | Strain secreting mRFP, parental strain for NID1596-NID1609 | This study |
NID1596 | argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN7679-TtrpC::AFpyrG | mRFP secretion, AN7679 overexpressed | Sc ERV41 ortholog | This study |
NID1597 | argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN2738-TtrpC::AFpyrG | mRFP secretion, AN2738 overexpressed | Sc ERV46 ortholog | This study |
NID1598 | argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN10724-TtrpC::AFpyrG | mRFP secretion, AN10724 overexpressed | Sc YIP3 ortholog | This study |
NID1599 | argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN11900-TtrpC::AFpyrG | mRFP secretion, AN11900 overexpressed | Sc BOS1 orholog | This study |
NID1600 | argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN0834-TtrpC::AFpyrG | mRFP secretion, AN0834 overexpressed | AN sec63 | This study |
NID1601 | argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN7302-TtrpC::AFpyrG | mRFP secretion, AN7302 overexpressed | Sc EMP47 ortholog | This study |
NID1602 | argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-ANRabD-TtrpC::AFpyrG | mRFP secretion, ANrabD overexpressed | AN rabD | This study |
NID1603 | argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-ANRabE-TtrpC::AFpyrG | mRFP secretion, ANrabE overexpressed | AN rabE | This study |
NID1604 | argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-ANSynA-TtrpC::AFpyrG | mRFP secretion, ANsynA overexpressed | AN synA | This study |
NID1605 | argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-ANSsoA-TtrpC::AFpyrG | mRFP secretion, ANssoA overexpressed | AN ssoA | This study |
NID1606 | argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-ANNsfA-TtrpC::AFpyrG | mRFP secretion, ANnsfA overexpressed | AN nsfA | This study |
NID1607 | argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN4759-TtrpC::AFpyrG | mRFP secretion, AN4759 overexpressed | Sc SEC2 ortholog | This study |
NID1608 | argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN10354-TtrpC::AFpyrG | mRFP secretion, AN10354 overexpressed | S.c. SEC11 ortholog | This study |
NID1609 | argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN6307-TtrpC::AFpyrG | mRFP secretion, AN6307 overexpressed | An02g04250 ortholog | This study |